Isolation, purification, characterization and application of alpha 1,5 endo L-arabinase
Alpha 1, 5- L endo-arabinase enzyme exhibits a number of industrial and biological applications which includes haze removal in wine and other beverages industry, anti-mycobacterial property, in production of cellulosic ethanol, paper and other food processing industries. The work started with the screening of effective producers of endo arabinase enzyme from different natural sources. We isolated about 78 different fungal isolates from different natural sources and from that through secondary screening we obtained effective 8 organisms which are active producers of endo arabinase enzyme and the enzyme was produced using specific media. Protein was precipitated using ammonium sulphate and purified by anion exchange chromatography. Protein bands were obtained in the 15% SDS gel corresponding to its molecular weight (. Enzyme was characterized and the kinetic study was done. During the study cost effective media was formulated for the production of endo arabinose.